VAV2 antibody (pTyr142)

Catalog No. ABIN7224890

This Rabbit Polyclonal antibody specifically detects VAV2 in WB, IF, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for VAV2 antibody (pTyr142) (ABIN7224890)

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VAV2 Antibody

Binding Specificity: pTyr142

Purpose: Vav2 (phospho Tyr142) Polyclonal Antibody

Specificity: Phospho-Vav2 (Y142) Polyclonal Antibody detects endogenous levels of Vav2 protein only when phosphorylated at Y142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Vav2 Phospho-Tyr142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: Vav2

Background: Rabbit Anti-Vav2 (phospho Tyr142) Polyclonal Antibody,VAV2, Guanine nucleotide exchange factor VAV2, VAV-2,VAV2 (vav guanine nucleotide exchange factor 2) is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for VAV2.,Guanine nucleotide exchange factor VAV2

Molecular Weight: observerd band 101kDa

Gene ID: 7410

UniProt: P52735

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway