VEGFR2/CD309 antibody (pTyr1175)

Catalog No. ABIN7212962

The Rabbit Polyclonal anti-VEGFR2/CD309 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect VEGFR2/CD309 in samples from Human, Mouse and Rat.

Quick Overview for VEGFR2/CD309 antibody (pTyr1175) (ABIN7212962)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFR2/CD309 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VEGFR2/CD309 Antibody

Binding Specificity: pTyr1175

Purpose: Flk-1 (phospho Tyr1175) Polyclonal Antibody

Specificity: Phospho-Flk-1 (Y1175) Polyclonal Antibody detects endogenous levels of Flk-1 protein only when phosphorylated at Y1175.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Flk-1 Phospho-Tyr1175

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: Flk-1

Background: Rabbit Anti-Flk-1 (phospho Tyr1175) Polyclonal Antibody,KDR, FLK1, VEGFR2, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD antigen CD309,Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR encodes one of the two receptors of the VEGF.Kinase insert domain receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of KDR are implicated in infantile capillary hemangiomas.,Vascular endothelial growth factor receptor 2

Molecular Weight: observerd band 152kDa

Gene ID: 3791

UniProt: P35968

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling