RECQL2 antibody (pSer1141)

Catalog No. ABIN7224946

The Rabbit Polyclonal anti-RECQL2 antibody (ABIN7224946) specifically detects RECQL2 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RECQL2 antibody (pSer1141) (ABIN7224946)

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RECQL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RECQL2 Antibody

Binding Specificity: pSer1141

Purpose: Rabbit Anti-WRN (phospho Ser1141) Polyclonal Antibody

Specificity: Phospho-WRN (S1141) Polyclonal Antibody detects endogenous levels of WRN protein only when phosphorylated at S1141.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human WRN Phospho-Ser1141

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RECQL2

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Alternative Name: WRN

Background: WRN, RECQ3, RECQL2, Werner syndrome ATP-dependent helicase, DNA helicase, RecQ-like type 3, RecQ3, Exonuclease WRN, RecQ protein-like 2WRN (Werner syndrome RecQ like helicase) encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in WRN are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Molecular Weight: 162kD

Gene ID: 7486

UniProt: Q14191

Pathways: DNA Damage Repair