Glutathione Reductase antibody (AA 44-522)

Catalog No. ABIN7232834

The Mouse Monoclonal anti-Glutathione Reductase antibody is suitable to detect Glutathione Reductase in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Glutathione Reductase antibody (AA 44-522) (ABIN7232834)

Target: Glutathione Reductase (GSR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutathione Reductase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2A2-1B7

Product Details anti-Glutathione Reductase Antibody

Binding Specificity: AA 44-522

Purpose: Anti-Glutathione Reductase Mouse Monoclonal Antibody

Specificity: These antibodies recognize human glutathione reductase. They do not cross-react with yeast glutathione reductase.

Cross-Reactivity: Human

Immunogen: Recombinant human glutathione reductase purified from E. coli expressing the human glutathione reductase gene (aa 44-522).

Isotype: IgG1

Application Details

Application Notes: ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3 μg/mL on the wells.ELISA Pairs: Both of these antibodies function as capture and detection antibodies at 1 μg/mL.
Immunoblotting: use at 0.5-1.0 μg/mL. A band of ~57 kDa is detected.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: These antibodies are stable for at least one (1) year at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details for Glutathione Reductase

Target: Glutathione Reductase (GSR)

Alternative Name: GSR

Background: Glutathione reductase, mitochondrial,Maintains high levels of reduced glutathione in the cytosol.,Glutathione reductase, also known as glutathione-disulfide reductase, is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. The importance of the glutathione pathway and enzymes that affect it is gaining an increased level of attention. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date.,[Isoform Mitochondrial]: Mitochondrion., [Isoform Cytoplasmic]: Cytoplasm.,GR, GRase, EC 1.8.1.7

NCBI Accession: NP_000628

UniProt: P00390

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis