ROR2 antibody (AA 2535-2835)

Catalog No. ABIN7232906

The Mouse Monoclonal anti-ROR2 antibody has been validated for WB and IHC. It is suitable to detect ROR2 in samples from Mouse.

Quick Overview for ROR2 antibody (AA 2535-2835) (ABIN7232906)

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ROR2 2535-2835

Product Details anti-ROR2 Antibody

Binding Specificity: AA 2535-2835

Purpose: Anti-ROR2 Mouse Monoclonal Antibody

Specificity: Mouse and human ROR2. Does not cross-react with ROR1.

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein G affinity chromatography

Immunogen: Bases 2535-2835 of mouse ROR2 fused in-frame to MBP and expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-10 μg/mL. A band of ~105 kDa is detected.
Immunohistochemistry: use at 1-10 μg/mL on paraformaldehyde-fixed, paraffin-embedded sections following antigen retrieval (microwave 12 min in citrate buffer, pH 6.0).
These are recommended concentrations.
Enduser should determine optimal concentrations for their application.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for ROR2

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Alternative Name: ROR2

Background: Tyrosine-protein kinase transmembrane receptor ROR2,Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development (By similarity). Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation (PubMed:17717073). In contrast, has also been shown to have very little tyrosine kinase activity in vitro. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443). {UniProtKB:Q9Z138, PubMed:17717073, PubMed:25029443}.,Receptor tyrosine kinases (RTKs) are cell surface receptors that regulate normal cellular processes through ligand-controlled tyrosine kinase activity. ROR2 is a membrane-bound RTK that is activated by Wnt signaling during normal bone and cartilage development. Recently, ROR2 has been shown to have pro-tumorigenic effects in osteosarcoma, melanoma, and renal cell carcinoma cell lines. ROR2 levels have also been described in soft-tissue sarcomas, specifically, in vitro studies revealed that invasive abilities of leiomyosarcoma (LMS) and gastrointestinal stromal tumor (GIST) are affected by ROR2 expression, and suppression of ROR2 reduces in vivo tumor mass in a xenotransplantation model of LMS.,Cell membrane, Single-pass type I membrane protein,EC 2.7.10.1, Neurotrophic tyrosine kinase, receptor-related 2

UniProt: Q01974

Pathways: RTK Signaling, WNT Signaling