Metabotropic Glutamate Receptor 5 antibody (C-Term)

Catalog No. ABIN7233043

This Mouse Monoclonal antibody specifically detects Metabotropic Glutamate Receptor 5 in WB, IHC, IP and IF/ICC. It exhibits reactivity toward Rat.

Quick Overview for Metabotropic Glutamate Receptor 5 antibody (C-Term) (ABIN7233043)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S75-3

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Binding Specificity: AA 824-1203, C-Term

Purpose: Anti-mGluR1/5 Glutamate Receptor Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat mGluR1 and mGluR5.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession no. P31424).

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting: use at 1 μg/mL. A band of ~130 kDa is detected. Smaller fragments due to proteolysis are detected as well.
Positive control: Rat brain lysate.
These are recommended concentrations. User should determine optimal concentrations for their application.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: Grm5

Background: Metabotropic glutamate receptor 5,G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium sPubMed:1320017, PubMed:21795692}.,The metabotropic glutamate receptors (mGluRs) are active through an indirect metabotropic process. They are members of the group C family of G-protein-coupled receptors, or GPCRs. Like all glutamate receptors, mGluRs bind glutamate which functions as an excitatory neurotransmitter. Metabotropic glutamate receptors are not ion channels. Instead, they activate biochemical cascades, leading to the modification of other proteins. This can lead to changes in the synapse's excitability. Eight different types of mGluRs, designated mGluR1 to mGluR8, are divided into groups I, II, and III. Receptor types are grouped based on receptor structure and physiological activity.,Cell membrane, Multi-pass membrane protein,mGluR5

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity