PINK1 antibody (C-Term)

Catalog No. ABIN7233046

This Mouse Monoclonal antibody specifically detects PINK1 in WB, IHC and IF/ICC. It exhibits reactivity toward Human.

Quick Overview for PINK1 antibody (C-Term) (ABIN7233046)

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PINK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S4-15

Product Details anti-PINK1 Antibody

Binding Specificity: AA 112-496, C-Term

Purpose: Anti-PINK1 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat PINK1.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Fusion protein corresponding to aa 112-496 (cytoplasmic C-terminus) of human PINK1. This sequence is 82

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. A band of ~50 kDa is detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their application.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least one (1) year at -20°C.

Target Details for PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Alternative Name: PINK1

Background: Serine/threonine-protein kinase PINK1, mitochondrial,PINK1 (PTEN-induced putative kinase 1) is a mitochondrial serine/threonine kinase that maintains mitochondrial function and integrity, provides protection against mitochondrial dysfunction during cellular stress, and is involved in the clearance of damaged mitochondria via selective autophagy. PINK1 is synthesized as a 63 kDa protein which undergoes proteolytic processing to yield two cleaved forms, ~55 kDa and ~42 kDa. PINK1 may be localized to the outer mitochondrial membrane with its kinase domain facing the cytosol which would explain the observed physical interaction between PINK1 and cytosolic E3 ubiquitin ligase Parkin.

UniProt: Q9BXM7

Pathways: Autophagy