MAPT antibody

Catalog No. ABIN7233074

The Mouse Monoclonal anti-MAPT antibody is suitable to detect MAPT in samples from Human. It has been validated for WB, ELISA, IF/ICC and DB.

Quick Overview for MAPT antibody (ABIN7233074)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (fixed cells) (IF/ICC), Dot Blot (DB)

Clone: 1D5

Product Details anti-MAPT Antibody

Purpose: Anti-Tau Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Tau.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Full-length recombinant human Tau441 (2N4R) P301S mutant protein pre-formed fibrils. In humans, mutation P301S gives rise to early-onset dementia.

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. A band of ~45.8 kDa is detected.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least one (1) year at -20°C.

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT

Background: Microtubule-associated protein tau,Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. {PubMed:21985311, PubMed:32961270}.,Cytoplasm, cytosol, Cell membrane, Cytoplasm, cytoskeleton, Cell projection, axon, Cell projection, dendrite, Secreted,Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

UniProt: P10636

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size