Adenosine A3 Receptor antibody (Extracellular) (FITC)

Catalog No. ABIN7237581

This Rabbit Polyclonal antibody specifically detects Adenosine A3 Receptor in FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for Adenosine A3 Receptor antibody (Extracellular) (FITC) (ABIN7237581)

Target: Adenosine A3 Receptor (ADORA3)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A3 Receptor antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Adenosine A3 Receptor Antibody (FITC)

Binding Specificity: AA 149-161, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): The antibody will not recognize adenosine A3 receptor from human samples.

Predicted Reactivity: Rat - 10 out of 13 amino acid residues identical

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GWNRKATLASSQN, corresponding to amino acid residues 149 - 161 of mouse ADORA3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7234643)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Adenosine A3 Receptor

Target: Adenosine A3 Receptor (ADORA3)

Alternative Name: ADORA3

Background: ADORA3, A3AR, Adenosine Receptor A3,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.ADORA3, A3AR, Adenosine Receptor A3

Gene ID: 11542

UniProt: Q61618

Pathways: Hormone Transport, cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process