GJC2 antibody (Intracellular Loop)

Catalog No. ABIN7886198

The Rabbit Polyclonal anti-GJC2 antibody (ABIN7886198) specifically detects GJC2 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for GJC2 antibody (Intracellular Loop) (ABIN7886198)

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GJC2 Antibody

Binding Specificity: AA 100-111, Intracellular Loop

Purpose: A Rabbit Polyclonal antibody to Connexin-47 (GJC2)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Intracellular loop

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235562)

Blocking Peptide: (ABIN7235562)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GJC2

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Alternative Name: GJC2 

Background:

Synonyms: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Description: Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.

Gene ID: 57165

NCBI Accession: NM_020435

UniProt: Q80XF7