PTAFR antibody (Extracellular)

Catalog No. ABIN7237758

The Rabbit Polyclonal anti-PTAFR antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect PTAFR in samples from Mouse.

Quick Overview for PTAFR antibody (Extracellular) (ABIN7237758)

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTAFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-PTAFR Antibody

Binding Specificity: AA 259-274, Extracellular

Purpose: A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)

Specificity: Extracellular, 3rd loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical,Human - 13 out of 16 amino acid residues identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7236256)

Blocking Peptide: (ABIN7236256)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PTAFR

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Alternative Name: PTAFR

Background: PTAFR, PAFR,Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1. PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2. Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.

Alternative names: PTAFR, PAFR

Gene ID: 19204

NCBI Accession: NM_000952

UniProt: Q62035

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin