TMC1 antibody (Extracellular, Loop 3)

Catalog No. ABIN7237916

This Rabbit Polyclonal antibody specifically detects TMC1 in WB, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for TMC1 antibody (Extracellular, Loop 3) (ABIN7237916)

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMC1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-TMC1 Antibody

Binding Specificity: Extracellular, Loop 3

Purpose: A Rabbit Polyclonal antibody to TMC1 (extracellular) 

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EHDFPSWMAKILR, corresponding to amino acid residues 679 - 691 of mouse TMC1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: 2.5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237509)

Blocking Peptide: (ABIN7237509)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TMC1

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Alternative Name: TMC1 

Background:

Synonyms: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1

Description: Transmembrane channel-like protein 1 (TMC1) is one of eight mammalian TMC genes. Proteins that belong to this family contain six transmembrane domains with both the C and N termini on the cytoplasmic side of the membrane, as well as a large loop between domains 4 and 5. This multi-pass transmembrane topologic structure suggests roles as a receptor, transporter, channel, or pump1. TMC1 and the closely related TMC2 gene are assembled as dimers2 and are expressed in neurosensory hair cells of the mouse inner ear where they appear to have a role as pore forming units in mechanoelectrical transduction. Mutations of the transmembrane channel-like 1 (TMC1) gene can cause dominant and recessive forms of deafness in humans and mice3 and hence, identification of the molecular functions of Tmc genes is broadly significant because it may provide a handle on the fundamental mechanisms that mediate human hearing and balance4.

Gene ID: 13409

NCBI Accession: NM_138691

UniProt: Q8R4P5

Pathways: Sensory Perception of Sound