Cullin 2 antibody

Catalog No. ABIN7266542

The Rabbit Monoclonal anti-Cullin 2 antibody has been validated for WB and IHC. It is suitable to detect Cullin 2 in samples from Human.

Quick Overview for Cullin 2 antibody (ABIN7266542)

Target: Cullin 2 (CUL2)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cullin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cullin 2 Antibody

Purpose: Cullin 2 Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: A synthesized peptide derived from human Cullin 2

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Cullin 2

Target: Cullin 2 (CUL2)

Alternative Name: CUL2

Background: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL mediates the ubiquitination of hypoxia-inducible factor (HIF. A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component are part of the DesCEND (destruction via C-end degrons pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins.,CUL2, cullin-2,Apoptosis,Cancer,Cell Biology & Developmental Biology,Cell Cycle,Cell Cycle_Cell cycle inhibitors,Ubiquitin,Ubiquitin_Ubiquitin-Proteasome Signaling Pathway,CUL2

Molecular Weight: 87kDa

Gene ID: 8453

UniProt: Q13617

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome