MAP3K3 antibody (AA 1-220)

Catalog No. ABIN7268652

This anti-MAP3K3 antibody is a Rabbit Polyclonal antibody detecting MAP3K3 in WB and IHC. Suitable for Human.

Quick Overview for MAP3K3 antibody (AA 1-220) (ABIN7268652)

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAP3K3 Antibody

Binding Specificity: AA 1-220

Purpose: MEKK3 Rabbit pAb

Sequence: MDEQEALNSI MNDLVALQMN RRHRMPGYET MKNKDTGHSN RQKKHNSSSS ALLNSPTVTT SSCAGASEKK KFLSDVRIKF EHNGERRIIA FSRPVKYEDV EHKVTTVFGQ PLDLHYMNNE LSILLKNQDD LDKAIDILDR SSSMKSLRIL LLSQDRNHNS SSPHSGVSRQ VRIKASQSAG DINTIYQPPE PRSRHLSVSS QNPGRSSPPP GYVPERQQHI

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MEKK3 (NP_976226.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:100 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for MAP3K3

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Alternative Name: MAP3K3

Background: This gene product is a 626-amino acid polypeptide that is 96.5 % identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively, it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,MAP3K3,MAPKKK3,MEKK3,Signal Transduction,Kinase,Serine/threonine kinases,MAPK-JNK Signaling Pathway,MAPK-P38 Signaling Pathway,Cell Biology & Developmental Biology,Cytoskeleton,Actins,Immunology & Inflammation,B Cell Receptor Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling,MAP3K3

Molecular Weight: 70kDa/74kDa

Gene ID: 4215

UniProt: Q99759

Pathways: MAPK Signaling