Recombinant DLG4 antibody (Alexa Fluor 647)

Catalog No. ABIN7272870

The Alpaca Monoclonal anti-DLG4 antibody has been validated for WB, IHC, ICC and IF. It is suitable to detect DLG4 in samples from Rat and Mouse.

Quick Overview for Recombinant DLG4 antibody (Alexa Fluor 647) (ABIN7272870)

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Rat, Mouse

Host: Alpaca

Clonality: Monoclonal

Conjugate: This DLG4 antibody is conjugated to Alexa Fluor 647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1B2

Product Details anti-DLG4 Antibody (Alexa Fluor 647)

Expression System: E.coli

Specificity: Recognizes mouse and rat PSD95. Other species not tested

Purification: Produced in: E.coli

Immunogen: anti-PSD95 is a fluorescently conjugated single-domain antibody that recognizes mouse and rat PSD95.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: IF: 1:500 (corresponding to 5 nM final concentration) , 5 µg/mL (IHC),

Comment:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: PSD95

Background: PSD95 is a member of the MAGUK family, and it plays an important role in the formation of a dynamic scaffold at the post synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity