mRuby antibody
Quick Overview for mRuby antibody (ABIN7273093)
Target
Reactivity
Host
Clonality
Conjugate
Application
-
-
Purpose
- anti-mRuby
-
Specificity
- In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
-
Cross-Reactivity (Details)
- Reacts against Red Fluorescent Protein
-
Characteristics
- Red Fluorescent Protein
-
Purification
- Epitope affinity purified
-
Immunogen
- Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
-
Isotype
- IgG
-
-
anti-mRuby antibody (Atto 488)
Reactivity: Entacmaea quadricolor IF, ICC, FACS Host: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
-
-
Application Notes
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 3 mg/mL
-
Buffer
- PBS, 20 % glycerol and 0.05 % sodium azide.
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- The antibody solution should be gently mixed before use.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
-
-
- mRuby
-
Background
-
Goat polyclonal antibody to mRuby (Red fluorescent protein). mRuby is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mRuby is a ~26 kDa protein that is optimally excited at a 558 nm and has a maximum of emission at 605 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
far-Red antibody.
-
Molecular Weight
- 26 kDa
Target
-
