KEAP1 antibody

Catalog No. ABIN7384390

This anti-KEAP1 antibody is a Rabbit Monoclonal antibody detecting KEAP1 in WB and IHC (p). Suitable for Human and Mouse.

Quick Overview for KEAP1 antibody (ABIN7384390)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R02-1H7

Product Details anti-KEAP1 Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000 IHC 1:20-1:50

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: Keap1

Background: Cytosolic inhibitor of Nrf2,INrf 2,INrf2,Keap 1,KEAP1,KEAP1,Kelch like ECH associated protein 1,Kelch like family member 19,Kelch like protein 19,Kelch-like ECH-associated protein 1,Kelch-like protein 19,KIAA0132,KLHL 19,KLHL19,MGC10630,MGC1114,MGC20887,MGC4407,MGC9454,KEAP1,also named as INRF2,KIAA0132 and KLHL19,is part of a multiprotein complex that contains the CUL3-ROC1 ubiquitin ligase,which can ubiquitinate the N-terminal domain of NRF2. Two molecules of KEAP1 bind to two distinct sites in the N-terminal region of NRF2,the ETGE and DLG sites,which affect the KEAP1-NRF2 interaction and/or its physiological consequences. KEAP1 retains NFE2L2/NRF2 in the cytosol. It functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It also retains BPTF in the cytosol. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human KEAP1.

Molecular Weight:

Observed_MW: 60/64kDa

Calculated_MW: 70kDa

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location