PCNA antibody

Catalog No. ABIN7384518

The Mouse Monoclonal anti-PCNA antibody has been validated for WB, IHC and IF. It is suitable to detect PCNA in samples from Zebrafish (Danio rerio).

Quick Overview for PCNA antibody (ABIN7384518)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 8B12

Product Details anti-PCNA Antibody

Purification: Protein A/G Purified

Immunogen: Recombinant human PCNA protein expressed by E.coli

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-2000, IP: 3 μg/sample, IF: 1:100-204

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: PBS with 0.1 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03,Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.

Molecular Weight:

Observed_MW: 36kDa

Calculated_MW: 30kDa

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy