ASCL2 antibody (C-Term)

Catalog No. ABIN7449050

The Rabbit Polyclonal anti-ASCL2 antibody has been validated for WB, IP, IHC and ChIP. It is suitable to detect ASCL2 in samples from Human.

Quick Overview for ASCL2 antibody (C-Term) (ABIN7449050)

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Product Details anti-ASCL2 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-ASH2 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 575 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:20,000

ChIP: 1 - 5 μg. Previous lots of this antibody have performed in this application.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ASCL2

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Alternative Name: ASH2

Background: Background: ASH2 (absent, small, or homeotic discs 2) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. ASH2 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX.

Gene ID: 9070

NCBI Accession: NP_004665

UniProt: Q9UBL3

Pathways: Stem Cell Maintenance