Recombinant TGFB1 antibody (AA 325-375)

Catalog No. ABIN7449529

This Rabbit Monoclonal antibody specifically detects TGFB1 in WB, IHC, ICC and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant TGFB1 antibody (AA 325-375) (ABIN7449529)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: BLR195J

Product Details anti-TGFB1 Antibody

Binding Specificity: AA 325-375

Purpose: Rabbit anti-TGF-beta 1 Recombinant Monoclonal Antibody [BLR195J]

Immunogen: between AA 325 and 375

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGF-beta 1

Background: Background: The Transforming growth factor beta-1 preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature TGF-beta 1 peptide. TGF-beta 1 bound to LAP is inactive, while the active form consists solely of the mature peptide homodimer. The mature TGF-beta 1 peptide may also form heterodimers with other TGFB family members. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. TGF-beta 1 regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells. [taken from NCBI Entrez Gene (Gene ID: 7040)].

Gene ID: 7040

NCBI Accession: NP_000651

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints