IWS1 antibody (AA 547-771)

Catalog No. ABIN7602008

The Rabbit Polyclonal anti-IWS1 antibody (ABIN7602008) specifically detects IWS1 in WB, ELISA and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for IWS1 antibody (AA 547-771) (ABIN7602008)

Target: IWS1 (IWS1 Homolog (IWS1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IWS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-IWS1 Antibody

Binding Specificity: AA 547-771

Purpose: Anti-IWS1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IWS1 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IWS1 recombinant protein (Position: R547-E771).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IWS1

Target: IWS1 (IWS1 Homolog (IWS1))

Alternative Name: IWS1

Background:

Background: Protein IWS1 homolog also known as interacts with Spt6 (IWS1) is a protein that in humans is encoded by the IWS1 gene. Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps. IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone. IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation. Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level .

Gene Full Name: interacts with SUPT6H, CTD assembly factor 1

Molecular Weight: 140 kDa

Gene ID: 55677

UniProt: Q96ST2