CSK antibody (AA 400-450)

Catalog No. ABIN7450141

This Rabbit Polyclonal antibody specifically detects CSK in WB and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for CSK antibody (AA 400-450) (ABIN7450141)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CSK Antibody

Binding Specificity: AA 400-450

Purpose: Rabbit anti-CSK Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 400 and 450

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: CSK

Background: Background: C-Src kinase (CSK) is a non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. CSK phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. CSK suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK [taken from the Universal Protein Resource (UniProt) P41240].

Gene ID: 1445

NCBI Accession: NP_004374

UniProt: P41240

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events