SPRY2 antibody (AA 265-315)

Catalog No. ABIN7451050

This Rabbit Polyclonal antibody specifically detects SPRY2 in IP. It exhibits reactivity toward Human.

Quick Overview for SPRY2 antibody (AA 265-315) (ABIN7451050)

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY2 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-SPRY2 Antibody

Binding Specificity: AA 265-315

Purpose: Rabbit anti-Sprouty 2 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Bovine

Purification: Affinity Purified

Immunogen: between AA 265 and 315

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7451050 has not performed satisfactorily when used for WB of Sprouty 2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Sprouty 2.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SPRY2

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Alternative Name: Sprouty 2

Background: Background: Sprouty 2 contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells sprouty 2 is transiently upregulated in response to fibroblast growth factor two. Sprouty 2 is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. Sprouty 2 interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. Sprouty 2 may play a role in alveoli branching during lung development as shown by a similar mouse protein [taken from NCBI Entrez Gene (Gene ID: 10253)].

Gene ID: 10253

NCBI Accession: NP_005833

UniProt: O43597

Pathways: EGFR Signaling Pathway, Sensory Perception of Sound, EGFR Downregulation