Neurofibromin 1 antibody (AA 150-200)

Catalog No. ABIN7451098

This Rabbit Polyclonal antibody specifically detects Neurofibromin 1 in IP. It exhibits reactivity toward Human.

Quick Overview for Neurofibromin 1 antibody (AA 150-200) (ABIN7451098)

Target: Neurofibromin 1 (NF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurofibromin 1 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-Neurofibromin 1 Antibody

Binding Specificity: AA 150-200

Purpose: Rabbit anti-NF1 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat

Purification: Affinity Purified

Immunogen: Between AA 150 and 200

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7451098 has not performed satisfactorily when used for WB of NF1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NF1.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Neurofibromin 1

Target: Neurofibromin 1 (NF1)

Alternative Name: NF1

Background: Background: Inactivating mutations in NF1 (neurofibromin 1) result in neurofibromatosis type I (NF1) which is characterized by Schwann cell neurofibromas, café-au-lait spots, and benign lesions of the iris. At the cellular level, NF1 functions as a negative regulator of Ras activity. Loss of NF1 leads to increased levels of active Ras-GTP which is important for the formation and maintenance of Schwann cell tumors.

Gene ID: 4763

NCBI Accession: NP_001035957

UniProt: P21359

Pathways: cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity