CDC27 antibody (AA 225-275)

Catalog No. ABIN7451689

This Rabbit Polyclonal antibody specifically detects CDC27 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for CDC27 antibody (AA 225-275) (ABIN7451689)

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC27 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CDC27 Antibody

Binding Specificity: AA 225-275

Purpose: Rabbit anti-CDC27 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 225 and 275

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: CDC27

Background: Background: CDC27 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions. CDC27 has been shown to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1.

Gene ID: 996

NCBI Accession: NP_001247

UniProt: P30260