His Tag antibody

Catalog No. ABIN7453371

The Goat Polyclonal anti-His Tag antibody (ABIN7453371) specifically detects His Tag in WB, ELISA, IP and ICC.

Quick Overview for His Tag antibody (ABIN7453371)

Target: His Tag

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-His Tag Antibody

Purpose: Goat anti-6-His Tag Antibody Affinity Purified

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes:

IP: 1 - 4 μg/mg lysate

ICC: 1:100 - 1:400

WB: 1:1,000 - 1:5,000

ELISA: 1:1,000 - 1:30,000, for coating plates 1:100 - 1:500

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate Buffered Saline (PBS) containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6-His

Target Type: Tag

Background: Background: The 6-His polyhistidine tag is a protein motif that consists of 6 or more histidine residues (H-H-H-H-H-H) engineered onto the N- or C- terminus of a protein of interest. Polyhistidine tags are useful for the detection and analysis of expressed recombinant proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.