Recombinant RPA2 antibody (pSer4, pSer8)

Catalog No. ABIN7453379

The Rabbit Monoclonal anti-RPA2 antibody has been validated for WB, IP, ICC and IF/ICC. It is suitable to detect RPA2 in samples from Human.

Quick Overview for Recombinant RPA2 antibody (pSer4, pSer8) (ABIN7453379)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (fixed cells) (IF/ICC)

Clone: BL-165-5F1

Product Details anti-RPA2 Antibody

Binding Specificity: pSer4, pSer8

Purpose: Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]

Immunogen: surrounding serine 4/8

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:1,000 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IP: 6 μL/1.0 mg lysate

ICC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA32

Background: Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA2, the 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

Gene ID: 6118

NCBI Accession: NP_002937

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA