CENPF antibody (AA 1800-1850)

Catalog No. ABIN7453389

This anti-CENPF antibody is a Rabbit Polyclonal antibody detecting CENPF in WB, IP and IF/ICC. Suitable for Human.

Quick Overview for CENPF antibody (AA 1800-1850) (ABIN7453389)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-CENPF Antibody

Binding Specificity: AA 1800-1850

Purpose: Rabbit anti-CENP-F/Mitosin Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1800 and 1850

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:2,000. Acetone fixation is recommended.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-CENP-F/Mitosin antibody A301-617A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENP-F/Mitosin

Background: Background: Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.

Gene ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection