HNF1A antibody (AA 581-631)

Catalog No. ABIN7453469

The Rabbit Polyclonal anti-HNF1A antibody has been validated for WB, IHC (fp) and IP. It is suitable to detect HNF1A in samples from Human.

Quick Overview for HNF1A antibody (AA 581-631) (ABIN7453469)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details anti-HNF1A Antibody

Binding Specificity: AA 581-631

Purpose: Rabbit anti-HNF1-alpha Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat

Purification: Affinity Purified

Immunogen: Between AA 581 and 631

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-HNF1-alpha antibody ABIN7453435.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1-alpha

Background: Background: Hepatocyte nuclear factor 1-alpha (HNF1-Alpha) is a transcription factor required for the expression of several liver-specific genes. HNF1-Alpha functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in the HNF1A gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas [taken from NCBI Entrez Gene (Gene ID: 6927)].

Gene ID: 6927

NCBI Accession: NP_000536

UniProt: P20823

Pathways: Hormone Transport, Carbohydrate Homeostasis