MAPKAP1 antibody (C-Term)

Catalog No. ABIN7453487

Product Details anti-MAPKAP1 Antibody

Target: MAPKAP1 (Mitogen-Activated Protein Kinase Associated Protein 1 (MAPKAP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-Sin1 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Chicken,Sheep,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 470 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MAPKAP1

Target: MAPKAP1 (Mitogen-Activated Protein Kinase Associated Protein 1 (MAPKAP1))

Alternative Name: Sin1 (MAPKAP1 Products)

Synonyms: CG10105 antibody, Dmel\\CG10105 antibody, dSin1 antibody, sin1 antibody, MAPKAP1 antibody, im:7143088 antibody, si:ch73-242f23.1 antibody, mip1 antibody, sin1b antibody, sin1g antibody, MGC53034 antibody, JC310 antibody, MIP1 antibody, SIN1 antibody, SIN1b antibody, SIN1g antibody, AI591529 antibody, D230039K05Rik antibody, Sin1 antibody, SAPK-interacting protein 1 antibody, mitogen-activated protein kinase associated protein 1 antibody, mitogen-activated protein kinase associated protein 1 L homeolog antibody, Sin1 antibody, MAPKAP1 antibody, mapkap1 antibody, Mapkap1 antibody, mapkap1.L antibody

Background: Background: Stress-activated map kinase-interacting protein 1 (Sin1) is highly similar to the yeast stress- activated protein kinase SIN1. Sin1 is an essential mTORC2 subunit that is required to act as the PDK2 that phosphorylates Akt/PKB at Ser 473. Sin1 has been implicated as a scaffolding protein. Sin1 is required for rictor binding in the mTORC2 complex. Sin1 has also been shown to be important to the SAPK (stress-activated kinase) signaling pathway and in the nucleus may act as a scaffold between ATF-2 and p38 to facilitate p38-induced phosphorylation of ATF-2. Sin1 is also found to form a complex with c-Jun N-terminal kinase (JNK) and may similarly function as a scaffolding protein in JNK signaling.

Gene ID: 79109

NCBI Accession: NP_001006618

UniProt: Q9BPZ7

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events