MCM3 antibody (AA 500-550)

Catalog No. ABIN7453566

The Rabbit Polyclonal anti-MCM3 antibody (ABIN7453566) specifically detects MCM3 in WB, IP and IHC (fp). The antibody is reactive with Human and Mouse samples.

Quick Overview for MCM3 antibody (AA 500-550) (ABIN7453566)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MCM3 Antibody

Binding Specificity: AA 500-550

Purpose: Rabbit anti-MCM3 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 500 and 550

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate. Use MCM2 antibody ABIN7453952 or MCM5 antibody to IP the MCM complex.

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background: Background: The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM3 has been shown to interact with MCM5, CDC46, and the acetyltransferase MCM3AP.

Gene ID: 4172

NCBI Accession: NP_002379

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA