MAD2 antibody (AA 100-150)

Catalog No. ABIN7453589

The Rabbit Polyclonal anti-MAD2 antibody has been validated for IP and IHC (fp). It is suitable to detect MAD2 in samples from Human.

Quick Overview for MAD2 antibody (AA 100-150) (ABIN7453589)

Target: MAD2

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MAD2 Antibody

Binding Specificity: AA 100-150

Purpose: Rabbit anti-MAD2 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 100 and 150

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-MAD2 antibody ABIN7450431.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MAD2

Target: MAD2

Background: Background: MAD2 (Mitotic Arrest Deficiency 2) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD2 is a subunit of the MCC complex that includes BubR1, Bub3, Cdc20 and Mad2, which may function to inhibit the APC/c (Anaphase Promoting Complex/Cyclosome). MAD2 is regulated by MAD1.

Gene ID: 4085

NCBI Accession: NP_002349

UniProt: Q13257