FBXW7 antibody (AA 50-100)

Catalog No. ABIN7453590

This anti-FBXW7 antibody is a Rabbit Polyclonal antibody detecting FBXW7 in IP and IHC (fp). Suitable for Human.

Quick Overview for FBXW7 antibody (AA 50-100) (ABIN7453590)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-FBXW7 Antibody

Binding Specificity: AA 50-100

Purpose: Rabbit anti-FBW7 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 50 and 100

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBW7

Background: Background: FBW7 (F-box and WD repeat domain containing 7) is a member of the F-box family of proteins. The F-box is a protein-protein interaction motif first identified in cyclin F. F-box proteins associate with SCFs (Skp1-cullin-F-box protein ligase complexes) which are E3 ligases that ubiquitinate proteins and target them for proteasomal degradation. FBW7 has been found to bind and specifically target cyclin E and the Notch intracellular domain (NICD) for ubiquitination. Defects in FBW7 may contribute to tumorigenesis.

Gene ID: 55294

NCBI Accession: NP_361014

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway