Apoptosis Inhibitor 5 antibody (AA 1-50)

Catalog No. ABIN7453657

This Rabbit Polyclonal antibody specifically detects Apoptosis Inhibitor 5 in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for Apoptosis Inhibitor 5 antibody (AA 1-50) (ABIN7453657)

Target: Apoptosis Inhibitor 5 (API5)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apoptosis Inhibitor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Apoptosis Inhibitor 5 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-API5 Antibody, Affinity Purified

Predicted Reactivity: X. laevis,Chicken,Orangutan

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Alternative Name: API5

Background: Background: API5 (apoptosis inhibitor 5) is an antiapoptotic factor that may have a role in protein assembly. API5 negatively regulates ACIN1. By binding to ACIN1, API5 suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. API5 is also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Gene ID: 8539

NCBI Accession: XP_508375

UniProt: Q9BZZ5

Pathways: Growth Factor Binding