Cyclin E1 antibody (AA 360-410)

Catalog No. ABIN7453685

The Rabbit Polyclonal anti-Cyclin E1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect Cyclin E1 in samples from Human.

Quick Overview for Cyclin E1 antibody (AA 360-410) (ABIN7453685)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Cyclin E1 Antibody

Binding Specificity: AA 360-410

Purpose: Rabbit anti-Cyclin E1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 360 and 410

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background: Background: Cyclin E1 is a regulatory subunit of the CDK2 kinase. Cyclin E is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis.

Gene ID: 898

NCBI Accession: NP_001229

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases