UBA2 antibody (AA 525-575)

Catalog No. ABIN7453775

Product Details anti-UBA2 Antibody

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Binding Specificity: AA 525-575

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-SAE2 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 525 and 575

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for UBA2

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Alternative Name: SAE2 (UBA2 Products)

Background: Background: SAE2 (SUMO-activating enzyme subunit 2) is also known as UBA2 (ubiquitin-like modifier activating enzyme 2). Heterodimers of SAE1 and SAE2 act as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. Heterodimers mediate ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 [taken from the Universal Protein Resource (UniProt) Q9UBT2].

Gene ID: 10054

NCBI Accession: NP_005490

UniProt: Q9UBT2