E2F1 antibody (AA 300-350)
Quick Overview for E2F1 antibody (AA 300-350) (ABIN7454373)
Target
See all E2F1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 300-350
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Purpose
- Rabbit anti-E2F1 Antibody, Affinity Purified
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Purification
- Affinity Purified
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Immunogen
- between AA 300 and 350
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Isotype
- IgG
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Application Notes
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IHC: 1:500 - 2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
IP: 2 - 5 μg/mg lysate
WB: Not recommended. Use rabbit anti-E2F1 antibody A300-766A.
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Restrictions
- For Research Use only
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Concentration
- 1000 μg/mL
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Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Expiry Date
- 12 months
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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Alternative Name
- E2F1
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Background
- Background: E2F1 is a member of the E2F family of transcription factors. E2F is an activating member that binds DNA at the E2 recognition site in the promoters of genes involved in cell cycle regulation or DNA replication. E2F1 is regulated through its interaction with the pRb tumor suppressor.
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Gene ID
- 1869
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NCBI Accession
- NP_005216
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UniProt
- Q01094
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Pathways
- p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
Target
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