LIG1 antibody (AA 200-250)

Catalog No. ABIN7454389

The Rabbit Polyclonal anti-LIG1 antibody has been validated for IP and IHC (fp). It is suitable to detect LIG1 in samples from Human.

Quick Overview for LIG1 antibody (AA 200-250) (ABIN7454389)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-LIG1 Antibody

Binding Specificity: AA 200-250

Purpose: Rabbit anti-DNA Ligase 1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 200 and 250

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA Ligase 1

Background: Background: DNA ligase 1 catalyzes the joining of double-stranded DNA ends during replication, recombination, and repair. Structurally, DNA ligase 1 is toroidal and encircles nicked DNA substrates. The 9-1-1 complex, a DNA damage checkpoint complex, has been reported to associate with DNA ligase 1 and stimulate ligase activity. Mutations in DNA ligase 1 result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Gene ID: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA