MYBL2 antibody (AA 250-300)

Catalog No. ABIN7454416

The Rabbit Polyclonal anti-MYBL2 antibody has been validated for IP and IHC (fp). It is suitable to detect MYBL2 in samples from Human.

Quick Overview for MYBL2 antibody (AA 250-300) (ABIN7454416)

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYBL2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MYBL2 Antibody

Binding Specificity: AA 250-300

Purpose: Rabbit anti-B-Myb Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. A301-654A has not performed satisfactorily when used for WB of B-Myb in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of B-Myb.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MYBL2

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Alternative Name: B-Myb

Background: Background: B-myb is transcription factor involved in multiple cellular processes such as cell survival, proliferation, and differentiation. B-myb was identified in a low-stringency screen of a T-cell cDNA library probed with a C-myb probe. C-myb is a cellular proto-oncogene highly conserved in vertebrates and invertebrates. The chicken c-myb proto-oncogene was the first ortholog discovered. Investigation of the origins of the avian myeloblastosis virus (AMV) led to the discovery that the AMV genome contained transduced sequences from normal chicken cellular DNA that resulted in a viral oncogenic version (v-myb) of the c-myb gene. B-myb is cell cycle regulated and expressed in all proliferating cells. Recent studies show that it is the only myb family member detected in embryonic stem cells and is essential to cell cycle progression and genomic stability.

Gene ID: 4605

NCBI Accession: NP_002457

UniProt: P10244

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Chromatin Binding