PDZ-RhoGEF (AA 125-175) antibody

Catalog No. ABIN7454424

This Rabbit Polyclonal antibody specifically detects PDZ-RhoGEF in IHC (fp) and IP. It exhibits reactivity toward Human.

Quick Overview for PDZ-RhoGEF (AA 125-175) antibody (ABIN7454424)

Target: PDZ-RhoGEF

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details

Binding Specificity: AA 125-175

Purpose: Rabbit anti-PDZ-RhoGEF Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 125 and 175

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-PDZ-RhoGEF antibody A301-952A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: PDZ-RhoGEF

Background: Background: PDZ-RhoGEF. (PDZ domain-containing Rho guanine nucleotide exchange factor) catalyzes the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. It contains a PDZ [post synaptic density protein (PSD95), Drosophila dis large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. PDZ-RhoGEF has been shown to be important to cell migration and the regulation of adhesion movement and trailing edge retraction.

Gene ID: 9826

NCBI Accession: NP_055599

UniProt: O15085