CDC25C antibody (AA 150-200)

Catalog No. ABIN7454534

This anti-CDC25C antibody is a Rabbit Polyclonal antibody detecting CDC25C in WB and IHC (fp). Suitable for Human.

Quick Overview for CDC25C antibody (AA 150-200) (ABIN7454534)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CDC25C Antibody

Binding Specificity: AA 150-200

Purpose: Rabbit anti-CDC25c Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 150 and 200

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25c

Background: Background: CDC25c (cell division cycle 25 homolog c) is a member of the Cdc25 family of phosphatases. CDC25c promotes cell cycle progression and M-phase entry by dephosphorylating CDC2/cyclin B and activating its kinase activity. CDC25c is phosphorylated and activated by multiple kinases including chk1, Cdc2/cyclin B, polo-like kinase 1, and JNK MAP kinases. The inactivation of CDC25c phosphatase activity is achieved through dephosphorylation by PP1 and PP2A phosphatases.

Gene ID: 995

NCBI Accession: NP_001781

UniProt: P30307

Pathways: Cell Division Cycle, M Phase