Cullin 4B antibody (AA 863-913)

Catalog No. ABIN7454566

The Rabbit Polyclonal anti-Cullin 4B antibody has been validated for WB and IHC (fp). It is suitable to detect Cullin 4B in samples from Human and Mouse.

Quick Overview for Cullin 4B antibody (AA 863-913) (ABIN7454566)

Target: Cullin 4B (CUL4B)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Cullin 4B Antibody

Binding Specificity: AA 863-913

Purpose: Rabbit anti-Cul4B Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 863 and 913

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Cullin 4B

Target: Cullin 4B (CUL4B)

Alternative Name: Cul4B

Background: Background: Cullin-4B is a member of the cullin family. Cullin-4B forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. Cullin-4B interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E [taken from NCBI Entrez Gene (Gene ID: 8450)].

Gene ID: 8450

NCBI Accession: NP_003579

UniProt: Q13620