CHMP2B antibody (AA 163-213)

Catalog No. ABIN7454583

This Rabbit Polyclonal antibody specifically detects CHMP2B in WB and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for CHMP2B antibody (AA 163-213) (ABIN7454583)

Target: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CHMP2B Antibody

Binding Specificity: AA 163-213

Purpose: Rabbit anti-CHMP2B Antibody, Affinity Purified

Predicted Reactivity: X. laevis,X. tropicalis,Chicken,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: Between AA 163 and 213

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CHMP2B

Target: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Alternative Name: CHMP2B

Background: Background: Charged multivesicular body protein 2b (CHMP2B) is a probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4 [taken from the Universal Protein Resource (UniProt) Q9UQN3].

Gene ID: 25978

NCBI Accession: NP_054762

UniProt: Q9UQN3