Topo IIalpha (C-Term) antibody

Catalog No. ABIN7454588

The Rabbit Polyclonal anti-Topo IIalpha antibody (ABIN7454588) specifically detects Topo IIalpha in WB, IHC (fp) and IP. The antibody is reactive with Human samples.

Quick Overview for Topo IIalpha (C-Term) antibody (ABIN7454588)

Target: Topo IIalpha

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details

Binding Specificity: C-Term

Purpose: Rabbit anti-Topo II Alpha Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1500 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:30,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Topo IIalpha

Alternative Name: Topo II Alpha

Background: Background: Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.

Gene ID: 7153

NCBI Accession: NP_001058

UniProt: P11388