DOT1L antibody (AA 1000-1050)

Catalog No. ABIN7454698

The Rabbit Polyclonal anti-DOT1L antibody has been validated for WB, IP and IHC (fp). It is suitable to detect DOT1L in samples from Human.

Quick Overview for DOT1L antibody (AA 1000-1050) (ABIN7454698)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-DOT1L Antibody

Binding Specificity: AA 1000-1050

Purpose: Rabbit anti-DOT1L Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1000 and 1050

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background: Background: DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.

Gene ID: 84444

NCBI Accession: NP_115871

UniProt: Q8TEK3