14-3-3 sigma/SFN antibody (AA 198-248)

Catalog No. ABIN7454767

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody has been validated for WB, IP and IHC (fp). It is suitable to detect 14-3-3 sigma/SFN in samples from Human.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 198-248) (ABIN7454767)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 198-248

Purpose: Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 198 and 248

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 Sigma

Background: Background: 14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.

Gene ID: 2810

NCBI Accession: NP_006133

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction