JAK2 antibody (AA 1082-1132)

Catalog No. ABIN7454809

The Rabbit Polyclonal anti-JAK2 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect JAK2 in samples from Human.

Quick Overview for JAK2 antibody (AA 1082-1132) (ABIN7454809)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-JAK2 Antibody

Binding Specificity: AA 1082-1132

Purpose: Rabbit anti-JAK2 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 1082 and 1132

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 15 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background: Background: JAK2 (Janus kinase 2) was originally cloned via screening of mouse cDNA libraries with PCR generated probes representing highly conserved elements in the protein tyrosine kinase (PTK) family. JAK2, as well as JAK, was named after the Roman god, Janus, the god of gates or doors, and who is depicted as having two faces which face opposite directions. JAK kinases bear two nearly identical phosphate transferring domains and function to transduce signals from type I and type II cytokine receptors for the activation of STAT (signal transducers and activators of transcription) transcription factors.

Gene ID: 3717

NCBI Accession: NP_004963

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response