SQSTM1 antibody (AA 375-425)

Catalog No. ABIN7454877

This Rabbit Polyclonal antibody specifically detects SQSTM1 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for SQSTM1 antibody (AA 375-425) (ABIN7454877)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 375-425

Purpose: Rabbit anti-Sequestosome-1 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Orangutan

Purification: Affinity Purified

Immunogen: between AA 375 and 425

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: Sequestosome-1

Background: Background: Sequestosome-1 is an adapter protein that binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Sequestosome-1 may play a role in titin/TTN downstream signaling in muscle cells, and may regulate signaling cascades through ubiquitination. Sequestosome-1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels [taken from the Universal Protein Resource (UniProt) Q13501].

Gene ID: 8878

NCBI Accession: NP_003891

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy