PSMD3 antibody (AA 1-50)

Catalog No. ABIN7454966

Product Details anti-PSMD3 Antibody

Target: PSMD3 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 3 (PSMD3))

Binding Specificity: AA 1-50

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-PSMD3 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: Between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PSMD3

Target: PSMD3 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 3 (PSMD3))

Alternative Name: PSMD3 (PSMD3 Products)

Synonyms: P58 antibody, RPN3 antibody, S3 antibody, TSTA2 antibody, AI255837 antibody, AntP91a antibody, D11Bwg1349e antibody, Psd3 antibody, Tstap91a antibody, proteasome 26S subunit, non-ATPase 3 antibody, proteasome 26S subunit, non-ATPase 3 S homeolog antibody, proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 antibody, 26S proteasome non-ATPase regulatory subunit 3 antibody, PSMD3 antibody, psmd3.S antibody, Psmd3 antibody, rpn-3 antibody

Background: Background: Proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3) is a subunit of the 26S proteasome, a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMD3 is a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid [taken from NCBI Entrez Gene (Gene ID: 5709)].

Gene ID: 5709

NCBI Accession: NP_002800

UniProt: O43242

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway