Recombinant ThPok (AA 250-300) antibody

Catalog No. ABIN7455169

The Rabbit Monoclonal anti-ThPok antibody is suitable to detect ThPok in samples from Human and Mouse. It has been validated for IP, WB, FACS, ICC and IHC.

Quick Overview for Recombinant ThPok (AA 250-300) antibody (ABIN7455169)

Target: ThPok

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Clone: BLR191J

Product Details

Binding Specificity: AA 250-300

Purpose: Rabbit anti-ThPOK Recombinant Monoclonal Antibody [BLR191J]

Purification: Purified

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details

Target: ThPok

Background: ZBTB7B is a transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. ZBTB7B is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is also a transcriptional repressor of the collagen COL1A1 and COL1A2 genes and may also function as a repressor of fibronectin and possibly other extracellular matrix genes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O15156].

Gene ID: 51043

UniProt: O15156